The maize and wheat MAD2 proteins are the only plant SAC proteins for which localisation to unattached kinetochores has been demonstrated [11], [12].
In plants, SAC protein homologs have been identified in silico [8] [10], but function has been investigated only for MAD2 for which localisation to unattached kinetochores has been demonstrated by immunolocalisation [11], [12].
This allows the approach to be implemented in challenging time-critical scenarios, such as in the localisation of artillery firing events, for which there may be only a small window of opportunity in which to perform a retaliatory strike.
The authors note that although the spatial rule is well established at the level of individual neurons in the SC, there are multisensory neurons located in many other areas of the brain involved in many different tasks for which stimulus localisation in space is possibly not vital.
This suggests an extracellular localisation for the proteins, which is typical of cell wall invertases [ 16].
Also plant species, for which a chloroplastic localisation of P5CR has been suggested deserve a sincere re-examination with respect to P5CR gene copy numbers and P5CR protein localisation [ 19, 20].
It is worth noting that a recent study using the budding yeast Saccharomyces cerevisiae, on the other hand, has challenged this model for which Aurora B localisation to the inner centromere is a prerequisite [ 30].
The method, already proposed for beam-type structures, has been extended to enable the detection and localisation of damage in plate-like structures for which only post-damage mode shapes are available.
Interestingly, we identified five gene clusters for which the average Gcn5 localisation pattern was representative of the majority of individual genes within the cluster (see Additional file 5).
Seven genes in the Gateway pcDNA-DEST47 C-terminal GFP fusion vector for which we have previously characterised sub-cellular localisations [ 7] were also included as transfection controls.
