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Localisation analysis revealed that antibodies to Escherichia coli-expressed recombinant As37 (rAs37) bound to muscle cells and the hypodermis.
Tagging with sequences encoding GFP or haemagglutinin (HA) epitopes allowed for identification and localisation analysis of 12 nuclear proteins that are potential regulators of P. falciparum gene expression.
Source localisation analysis of our data supported the existence of separate generators in auditory cortex: a tangentially-oriented one that showed substantial developmental change between 7 and 11 years, and a radially-oriented one that did not show age changes.
In U. maydis, a genome-wide localisation analysis of all chitin synthases using GFP fusion proteins indicated that some of these chitin synthases were co-localized with the endosomes, suggesting that chitin synthases are good candidates for recycling processes at the growing hyphal tips [13].
Localisation analysis has been described elsewhere [ 21].
Protein localisation analysis using WoLF PSORT [ 64] and BLAST matches were used to support each prediction.
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A statistical analysis of the images was performed using ImageJ statistical plugin JACoP, a tool commonly used for co-localisation analysis.
Co-localisation analysis was performed using Imaris (Bitplane).
For co-localisation analysis, 48 h infected myocytes were loaded with MitoTracker deep red (500 nM; Invitrogen) for 20 min at room temperature (approximately 21 °C) and washed.
In contrast to the cytoplasmic expression of FGF2 in cancer cells, many (∼35%) myo-fibroblasts (activated PSCs (Apte et al, 1999)) expressed nuclear FGF2, as evident from co-localisation analysis (Supplementary Fig 1, Fig 1B).
Cellular co-localisation analysis of the two luminescence signals reveals poor overlap of 2 and DAPI, indicating that the RuII complex does not strongly co-localize with the DNA dye (Fig. 5c and d). 1 was employed as a positive control for cellular DNA targeting and the strong co-localization between DAPI emission and the MLCT of 1 is evident (Fig. S2 †).
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