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Briefly, ten single nucleotide variants and one local deletion were selected at random for chain termination sequencing (Sanger method).
The specific role of HIF-2α was determined via local deletion of HIF-2α in joint tissues or using conditional knockout (KO) mice.
Local deletion of HIF-2α in joint tissues inhibited pannus formation adjacent to cartilage tissue and cartilage destruction caused by K/BxN serum transfer.
Local deletion of HIF-2α in joint tissues via IA injection of Ad- Cre in Epas1fl/fl mice suppressed pannus formation and cartilage destruction.
Given such an arrangement, one local deletion could join the two together with a one in three chance of maintaining the reading frame.
Local deletion of the GHR in the liver may serve as a very useful experimental animal model for analyzing the role of tissue-specific disruption of GH signaling in the control of processes observed in living organisms.
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Furthermore, the SB system offers the possibility of transposase-induced excision out of the genome to induce local deletions or to revert possible mutant phenotypes.
The first two relied on mobilizing excisions of existing P-element transposon insertions at the Lerp locus to generate local deletions within Lerp.
However, we observed that in 43% of re-mobilizations, inaccurate excisions occurred and led to various mutations in the original locus, including local deletions, insertions, and indels.
We anticipate that double strand breaks induced by I-SceI meganuclease may be repaired by error-prone repair mechanisms, leading to generation of local deletions large enough to remove one or more coding exons of the mutated gene.
A tandem duplication of Cdic (cytoplasmic dynein intermediate chain) and AnnX (annexin X) followed by three local deletions joined several exons of these two genes together to create the novel gene Sdic[ 38].
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