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Results show that loads can be expected that are substantially lower than the external loads on the roof surface, as given in wind loading standards.
The quasi-steady design approach is used by many wind loading standards such as AS1170.2 1989, to determine the design net (i.e. external internal) pressures on cladding elements and fixtures of low-rise buildings.
The level of the actA and gdh2 genes that are commonly used loading standards for Northern analysis varied by less than 21%.
The level of the actA [ 38] and gdh2 transcripts, which are commonly used loading standards for.Northern analysis, varied by less than 14% over the four situations tested.
As this normalization method might not be appropriate for dynamic cultivation conditions, we considered transcript levels of a few so-called 'house-keeping' genes commonly used as loading standards for Northern analysis and quantitative RT-PCR.
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β-Actin was used as loading standard.
In the United States, a new chapter has been developed for the ASCE 7 loading standard to design important buildings and other structures for tsunami loads and their effects.
As internal loading standard, membranes were stripped and stained with Coomassie Blue R-250.
Detection of actin in the honey bees was used as a positive indicator of intact mRNA being assayed and also serves as an internal loading standard.
Mouse monoclonal anti-chicken actin (Abcam Inc., Toronto, ON, Canada) was used as a loading standard.
The qPCR experiments were run on a Stratagene Mx3000P Real-Time System (La Jolla, CA, USA) using SYBR green I with ROX as an internal loading standard.
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