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Actin served as a control for equal loading of proteins.
Ponceau staining of membranes confirmed equal loading of proteins.
Equal loading of proteins was validated by incubation of the Western blot with an anti-tubulin antibody (Labvision, Fremont, CA).
Equal loading of proteins on the gel was confirmed by probing the blots for β-actin or Oct-1.
Loading of proteins was performed during strip rehydration with the recommended volume (450 µl for 24 cm strips) over night.
Comparable loading of proteins was verified by reprobing the blots with an antibody specific for the housekeeping gene product, GAPDH.
Similar(19)
Levels of β-actin confirmed equal loading of protein samples.
β-actin was used as a standard for the equal loading of protein in the lanes.
Actin levels were measured to ensure equal loading of protein.
Equal loading of protein in all experiments was confirmed by Coomassie blue staining of blots.
Equal loading of protein was confirmed by the detection of similar intensity β-actin bands.
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