Exact(4)
Bradford reagent (Sigma) was used to ensure equal loading of lysates according to the manufacturer's recommendations.
Equal loading of lysates is demonstrated by GAPDH expression.
Parallel samples were probed using an anti-actin antibody (Chemicon, Billerica, MA, USA) to confirm the equal loading of lysates between lanes.
This difference can readily be seen in Figure 8d, in which equal loading of lysates shows no detectable level of Bim EL in T-47D cells compared with readily detectable Bim EL expression in MCF-7 cells.
Similar(56)
(A ) Western blot of Sec31, Sec23, and tubulin (loading control) of lysates from GFP, Sec16, Sec23, and Sec24AB-depleted cells grown in Schneider's (S) and incubated with KRB for 4 hr (K).
Equal loading of total lysates was confirmed by GAPDH.
Equal loading of total lysates was confirmed with GAPDH.
To ensure equal loading of cell lysates, β-actin was used as a control.
Equal loading of cell lysates was assessed by a mouse anti-β-Actin antibody (Sigma-Aldrich).
β-actin was used as intra-assay marker for both quality and quantity loading of sample lysates.
As a control for equal loading of protein lysates, the blotted proteins were probed with antibody against anti-γ-tubulin protein.
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