Sentence examples for loading of lanes from inspiring English sources

Blots were stained with Ponceau S to verify equal loading of lanes.

To verify equal loading of lanes, blots were stripped and reprobed with anti-tubulin (Sigma Aldrich).

An anti-glyceraldehyde-3-phosphate dehydrogenase antibody (Chemicon International Inc., Temecula, CA, USA) was used to control equal loading of lanes.

An anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Chemicon International, Temecula, CA, USA) was used to control equal loading of lanes.

Thirty μg of total protein for each sample were separated on a 10% or 15% SDS-PAGE gel, transferred onto Hybond nitrocellulose membranes (Amersham Biosciences, Piscataway, NJ) using standard conditions, and stained with Ponceau S to verify transfer and equal loading of lanes.

To account for possible inconsistencies in staining across the gel and loading of the lanes, all bands were quantified as a fraction of the total DNA loaded in a lane.

After electrotransfer, the nitrocellulose membrane (Protran, Schleicher & Schuell, Dassel, Germany) was stained with Ponceau S (PonS) in order to control complete transfer and equal loading of the lanes.

Equal loading of gel lanes was confirmed by hybridization with the house keeping gene 1B15.

Antibody to β-actin (Table 1) was used to control for equal loading of gel lanes.

Equal loading of all lanes was confirmed by reprobing the membranes with anti-β-actin antibody (Sigma, St . Louis MO).

After analysis, each blot was stripped and incubated with a mouse anti-human mouse anti-humanin antibody (1 : 5000 final dilution) to ascertain equivalent loading of the lanes.