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The gel loading mixture was prepared by adding 5% Coomassie Blue Brilliant G-250 dissolved in 1 M aminocaproic acid to the sample at a ratio of 1∶6 (dye:sample volume).
The loading mixture has been denatured at 95°C for 3 min, followed by chilling on ice for 3 min immediately.
One μl of the diluted sample was added to 9.1 μl of loading mixture made up with 9 μl HiDi formamide and 0.1 μl Genescan 500 LIZ internal size standard (Applied Biosystems).
Five μL of Formamide Loading Dye (GE Healthcare Life Sciences) were added to each DNA sample and the loading mixture was concentrated for 50 min at 65°C up to a volume of approximately 5 μL.
Three μl of each PCR product was added to 20 μl of water, 1 μl of the diluted sample was added to 12.5 μl of loading mixture made up with 12 μl of HiDi formamide and 0.5 μl of Genscan 400 ROX internal size standard.
Three microliters of each PCR product were added to 20 μL of water, 1 μL of the diluted sample was added to 12.5 μL of loading mixture made up with 12 μL of HiDi formamide and 0.5 μL of Genscan 400 ROX internal size standard.
Similar(53)
Inspection of response variations, especially in the S-parameter Smith chart and in the neighborhoods of resonance, allows the discrimination of different loading mixtures.
Two different nanoemulsions of mean droplet size below 600 nm were produced, loading mixtures of natural or synthetic pentacyclic triterpenes, respectively.
The formulation loading a mixture of natural triterpenes showed greater ability to inhibit inflammation than that loading the synthetic mixture.
Voids filled with asphalt (VFA) are proposed as a parameter to model bleeding of asphalt concrete, and its variation for different loading and mixture condition is studied.
The first type was prepared by loading the mixture of OCT/PLGA into TNTs.
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