Sentence examples for loading mix from inspiring English sources

The fluorescent-labelled PCR samples were prepared for electrophoresis by combining 1 μL PCR product with 14 μL loading mix (13.5 μL Hi-DI formamide, 0.5 μL of in-house prepared ROX size standard, [ 54]).

Briefly, the PCR mixture contained 12.5 μl of 2 × Premix Taq (loading mix) (TaKaRa Biotech Corporation, Dalian, China), 1 μl each of forward and reverse primers, 5 μl of the cDNA, and 5.5 μl of ultrapure water.

For the CE-SSCP analyses, the fluorescent-labelled PCR samples were prepared for electrophoresis by combining 1 μl PCR product with 14 μl loading mix which consisted of 13.75 μl Hi-DI formamide and 0.25 μl Genescan ROX 350 standard (Applied Biosystem).

Culture supernatant was fractionated by sequential centrifugation at 2200 g (10 min at 3200 rpm) and 100 000 g (60 min at 30 000 rpm) for 10 min. The final supernatant was discarded and the pellet lysed in SDS-PAGE gel loading mix prior to SDS-PAGE analysis using a 3 cm, 4% stacking gel and a 12% resolving gel.

On the other hand, increasing cobalt loading, mixing speed and space velocity shift the products towards heavier compounds.

The consecutive loading, mixing, and aliquoting of the LAMP primers/reagents and DNA sample solutions were accomplished using an optimized square-wave microchannel, metering chambers and revulsion per minute (RPM) control.

The slight differences observed are likely attributed to the solid loading, mixing and cellulose regeneration method at the larger scale.

Incoming pigs may appear healthy but be incubating infection or acting as carriers of a pathogen, and the likelihood of transmission to susceptible pigs is increased by the stress inherent to loading, mixing, and transportation of these animals [ 12, 13].

Once the samples and assays inlets were loaded, the 136x load mix script was executed to load the samples and assays.

FALSTAFF, TWIST and variations of these spectra have been interrogated using mix counting algorithms to illustrate the level of load mixing that occurs in these standard spectra.

This load-mix process takes approximately 45 minutes.