Sentence examples for loading and transfer from inspiring English sources

Equal loading and transfer efficiency were verified with Ponceau Red staining.

An anti-mGluR1a mouse polyclonal antibody (Upstate Cell Signaling Solutions) was also used and a rabbit anti-calnexin polyclonal primary antibody (Stressgen, Victoria, British Columbia, Canada) indexed protein loading and transfer.

Such a change would allow for a faster, safer and simplified operation with automated loading and transfer to an accompanied automated chemistry module.

Ponceau S staining was used to evaluate protein loading and transfer in all Western blots.

The membranes were stained with 0.2% Ponceau S red to assure equal protein loading and transfer.

α-tubulin antibody was used to control for equal loading and transfer of the samples, as mentioned previously.

Equal protein loading and transfer was confirmed by staining the membrane with Ponceau Red [0.2% (w/v) in H2O:acetic acid 99∶1].

Experiments involving immunoblotting procedures were repeated at least three times and blots were re-probed with an antibody for β-actin to control for protein loading and transfer.

Equality of protein loading and transfer onto membranes was confirmed by probing for β-actin and staining with Ponceau S solution (Sigma).

Blots of mitochondrial proteins were confirmed for equal loading and transfer using MemCode reversible protein stains (Pierce Biotechnology, Rockford, IL, USA).

Membranes were blocked with 5% milk and then incubated with α-tubulin antibody (B-7 sc-5286, mouse monoclonal 1∶600, Santa Cruz Biotechnology, CA) to control for equal loading and transfer of the samples.