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The 4 12% Bi Tris gradient gels (for proteins above 20 kDa) or 12% Bis Tris gels (for histones and histone marks) were loaded with samples supplemented with Roti-Load 1 sample buffer.
Supplements loaded with vitamin blends and antioxidants can help keep those nasty headaches at bay.
To analyze whether TPEN treatment perturbed intracellular zinc content, human eggs were first loaded with 10 μM Fluozin3-AM supplemented with 0.02% pluronic acid for 30 min at room temperature and then the eggs were incubated in CZB medium (Millipore, Temecula, CA) containing 0.5% DMSO with or without 50 μM TPEN for 30 min at 37 °C in an atmosphere of 5% CO2 in air.
T cells were loaded with Rhod-2 in HBSS buffer supplemented with 0.02% pluronic acid F-127 for 30 min at 37°C, washed with phosphate buffered saline (PBS) buffer 3 times, and then incubated in the PBS buffer for 2 h at room temperature to allow for complete deesterification.
Add omega-3s to your diet by either taking a supplement or eating fish that are loaded with omega-3 fatty acids, like salmon, tuna and trout.
To validate these results independently, cells were loaded with an oxygen-sensitive probe (MitoXpress; Axxora, San Diego, CA) in media supplemented with palmitic acid and BSA.
Cells were loaded with 5 μM Fluo-4/AM in HBSS (Hanks balanced saline solution) supplemented with 2.5 mM probenecid for 45 min and then washed twice with HBSS.
Addition of thapsigargin in Ca2+-free medium (supplemented with 15 mM EGTA) resulted in an increase of fluorescence in RBCs of T. torquatus loaded with Fluo-3 AM.
Or are loaded with blanks.
Each is loaded with batteries.
"He is loaded with contradictions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com