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Following preparation of sufficiently high quality total RNA, samples were labelled, loaded onto the array slides and read following the manufacturer's instructions.
A MAUI Mixer FL Hybridization Chamber Lid (BioMicro Systems, Salt Lake City, UT, USA) was adhered onto the slide to form a sealed chamber, allowing 46 µl of target to be loaded onto the array.
Samples were loaded onto the array and hybridized at 55°C for 20 hours.
Next, sample cocktails were loaded onto the array and amplification was performed as recommended by the vendor.
For each sample, a mixture of TaqMan Gene Expression Master Mix and cDNA equivalent to 250 ng total RNA was loaded onto the array and subjected to the amplification of 93 immune- or inflammation-related genes plus three endogenous controls.
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Each sample (750 ng) was loaded onto the arrays.
The DNA was denatured for 10 min at 95°C and then kept at 42°C until it was loaded onto the arrays.
The samples were then immediately hybridized onto HT-12_v3_BeadChips; 750 ng of each sample was loaded onto the arrays.
Biotin-labelled cRNA was prepared using the BioArray High Yield RNA Transcript Labelling Kit (Enzo, Farmingdale, NY, USA), and 15 μg was loaded onto the probe array cartridge (Bovine Genome Array; Affymetrix, Santa Clara, CA, USA).
15 μg of cRNA was loaded onto the probe array cartridge of Bovine Genome Array (Affymetrix Clara CA, USA).
The DNA was loaded onto the nanochannel array of BioNano Genomics IrysChip by electrophoresis of DNA.
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