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Another concern for the LoAD system is cytotoxicity of MS2-CtIP.
Furthermore, we created various CtIP mutants previously characterized, and analyzed their functionality in the LoAD system (Supplementary Fig. 10).
Using this local accumulation of DSB repair molecules (LoAD) system, strong pathway choice at the CRISPR target site should be achieved (Fig. 1a).
To further expand the application of the LoAD system, we additionally examined the enhancement of other types of genome editing by accumulating various effector proteins.
Further possibilities of the LoAD system will be revealed by large-scale effector screening, contributing to much more biased, controllable, and precise genome engineering.
In this study, we attempt to enhance the efficiency further to secure robust gene knock-ins, by using the local accumulation of DSB repair molecules (LoAD) system.
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He said eye-tracking technology had originally been developed largely with military funds to create a look-and-load system for fighter pilots.
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Besides, a single stove cannot enforce the thermal comfort during design weather conditions: a peak-load system is then needed.
In this paper we present a multi-level and distributed control system, based on a robust Model Predictive Control MPCC) technique, for a multi-body slung-load system.
In this paper, a new oriented control model for SAPF-load system, taking into account for the nonlinearity of coil characteristics, is developed.
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