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Load samples (normally 10 µL) directly into the wells.
Duplicate cervical and vaginal viral load samples (n = 301) were taken at four stages (menstrual, follicular, periovulatory, and luteal) of two consecutive cycles.
Phylogenetic analysis of MERS-CoV has occurred by analysis of full-length genomes or multiple concatenated genome fragments, which is time-consuming, costly and limited to high viral load samples.
Low viral load samples that generated total copy CTs which appeared after 26 cycles sometimes yielded false-positive results.
The Νanoflex ™ 4-IFC Controller utilizes pressure to control the valves in the chips and load samples and gene expression assay reagents into the reaction chambers.
Detection was correlated to input viral load since the very low load samples remained negative in VIDISCA-454 (median viral genome concentration in VIDISCA-negative samples 3.5 E3; range 6.0 E2–1.1 E5).
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Sampling of this multi-state generator is given in Fig. 3, similar to the load sampling [17].
Load sample.
Load sample on an agarose gel.
The user interface also allows the user to load sample data and network for analysis.
The viral load sampled during day 1 to day 3 was significantly higher than the viral load sampled on day 4 or later (median 3.68 vs. 3.13 log10 copies/mL, p = 0.01 with Mann–Whitney U test).
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