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In summary, a quantitative RT-RPA on LOAD assay with a high level of sensitivity was developed as a foundation for further developments in medical bedside and POC diagnostics.
The Cavidi viral load assay and the ultra-sensitive p24 antigen assay (Up24 Ag) have been suggested as more feasible alternatives to PCR-based HIV viral load assays for use in monitoring patients infected with HIV-1 in resource-limited settings.
Plasma HIV-2 viral load has been reported as predictive of AIDS in HIV-2 infected patient but the lack of sensitivity of the current HIV2 viral load assay is a limitation for the monitoring of the HIV-2-infected patients.
The results of the viral load assay correlate with the levels of DENV NS1 protein in plasma (Fig. 1B).
Results for the bacterial load assay and specific organism assays are shown in Figures 2a and 2b, respectively.
However, a small number of LTNPs exhibited suppression of viral replication to levels below the detection threshold of the viral load assay.
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Several commercially available HIV-1 viral load assays based on real-time detection technology and automated platforms are available.
HIV-1 viral load assays are critical tools to monitor antiretroviral therapy efficacy in HIV-infected patients.
The recent introduction of highly sensitive viral load assays resulted in a significant increase in number of treated HIV-infected patients with a detectable viral load.
Due to costs and lack of currently available commercial viral load assays, insufficient attention has been paid to therapy monitoring through measurement of plasma viral load.
Plasma human immunodeficiency virus type 1 (HIV-1) RNA level is an important parameter for patient management, yet viral load assays from different manufacturers are not standardized.
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