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Our results show that the levels of IDO mRNA in Bhsp65-restimulated LNC of TNFα-treated rats (1.51 fold compared to LNC in medium) were comparable (p > 0.05) to that of PBS-treated rats (2.26 fold).
Immunonanocapsules were synthesized by conjugation to lipid nanocapsules (LNC) of whole OX26 monoclonal antibodies (OX26 MAb) directed against the transferrin receptor (TfR).
Lymph node cells (LNC) of the vaccinated rats were incubated with irradiated activated or resting A2b T cells, and proliferative responses were measured to different numbers of A2b stimulator cells.
LNC of rats with actively induced AA were prepared and activated with Mt176-90 in the presence of the Anti-p277 anti-ergotypic T-cell line, or in the presence of the control Anti-MBP T-cell line.
For DLI, LNC (of which approximately 60% were CD3+ T cells, data not shown) were obtained from mesenteric and cervical lymph nodes of 10-12 w-old mand and female PVG.7B rats by filtering through cell strainers (BD Biosciences).
Neither the transfer of Mtb-primed LNC of WKY rats into LEW recipients nor the transfer of Mtb-primed LNC of LEW rats into WKY recipients resulted in retention of T cells in the hind paws of recipient rats.
Similar(48)
LNCs of Celastrus-treated or control rats were plated in a 96-well plate as for the LNC proliferation assay described above.
We tested the draining LNCs of arthritic rats ex vivo as well as after their restimulation with Bhsp65.
Taken together, altered expression of the genes related to Th1 and Th17 responses is the most predominant change following Bhsp65 restimulation of LNCs of preclinical arthritis rats.
The draining LNCs of LEW rats (with or without the tolerogenic Bhsp65 pretreatment) were collected on day 7 after Mtb immunization.
In this context, we examined the expression profile of Bhsp65-induced genes in the draining LNCs of LEW rats in the Inc phase of AA.
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