Sentence examples similar to lm Kit from inspiring English sources
ApoAlert LM-PCR ladder assay kit (CLONTECH) was used following the manufacturer's instructions as summarized below.
The first, second, and third checkpoint are gathering learner maps (LM) and assessing the learner maps (AS) by using Kit-Build concept map.
The amplified product from the second LM-PCR was purified using a QIAquick PCR Purification Kit (Qiagen) according to the manufacturer's instructions with minor modifications.
The following modifications were made: For the RNeasy Kit (Qiagen) we used a combination of enzymatic lysis (LM solution) and mechanical cell disruption by vortexing with zirconia beads (0.1 mm) instead of bead-beating.
After LM-PCR, the reactions were cleaned up using a QIAquick PCR Purification kit (Qiagen) and eluted with 50 μl of water (pre-heated to 50°C).
Subsequently, the LM-PCR products were cloned into Illumina libraries using the Genomic DNA Sample Preparation Kit for paired end sequencing with the HiSeq 2000 or the MiSeq platforms.
4 µg of the RE33150 EST clone of lms, obtained from the Riken embryo library, were digested with EcoRI, gel purified with the QIAquick Gel Extraction Kit (Qiagen) and resuspended in 30 µl of DEPC-H2O.
Because the total RNA quantity of LM-captured epidermal cells was about 10 ng, we used a TargetAmp two-round aminoallyl-aRNA (antisense RNA) amplification kit (Epicentre Biotechnologies, Madison, WI, U.S.A).
