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The PCR product (300 ng) was digested with 1-2 Units of BsmFI restriction enzyme (New England Biolabs, Foster City, CA, USA) and 1 ·reaction buffer B in a 30 lL reaction volume.
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A DAP variant has been recently identified in this plant that utilizes a novel transaminase (LL-aminoadipate aminotransferase, the product of ORF At4g33680) that specifically catalyzes the conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction requiring three enzymes in the DAP-pathway variant found in E. coli [ 32].
Based on assumption (4), if ( k_{text{mt,A}} ll k ) in the initial reaction period: k_{text{app}} = k_{text{mt,A}} (12).
LL and BL patients who developed reaction during the course of the disease were also included in the study.
In most of the region of the disk, (rho _d ll rho _g), and the back-reaction of migrating dust is not effective for erasing the pressure bump.
For the RT-PCR amplification, 0.5 lL of cDNA was used for each reaction with different primer pairs (primer sequences are listed in Table 1).
Interestingly, these levels decreased dramatically (R LLENL; P < 0.05) during reaction, reaching values similar to those detected in the NR LL group, as observed in Figure 2c, d.
Taken together, these results demonstrate that the IGF-I and IGFBP-3 circulating levels at disease diagnosis were significantly higher in the LL patients who developed ENL than among the LL patients who did not experience reaction during MDT.
The reaction velocity of trypsin with chromogenic substrate in the presence of LL-TIL was shown in Table 1.
These results showed that the deepoxidation reactions from Vx to Ax and Zx and the epoxidation reactions from Ax and Zx to Vx occur under HL and LL, respectively.
Conjugation reaction was performed for 3 h at RT and stopped by addition of LL-Quencher FD reagent.
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