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The baby: age, sex, breastfeeding, medical history, living nucleus.
The father and/or mother will be asked to provide, using an electronic data collection system created for the study, data regarding the following: 1-The baby: age, sex, breastfed/artificial milk, living nucleus, medical history and clinical data for follow-up, 2- The parents: age, sex, socio-demographic data, smoking status (yes or no).
The living nucleus makes this a valuable research tool in the study of chromatin structure, function, and dynamics.
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After treatments, proteins were crosslinked to DNA in living nuclei by adding formaldehyde directly to the cell culture medium to a final concentration of 1%.
Protein complexes were cross-linked to DNA in living nuclei by adding 1% of formaldehyde (Merck, Whitehouse Station, NJ, USA) to the culture medium for 10 min at room temperature.
The normalized hemoglobin content and live nucleus numbers were determined separately to evaluate the angiogenesis and viability of transplanted hepatocytes.
The first day after incubation, 0.5 μL of Hoechst 33342 stock solution was added into each well for living cell nucleus staining 30 min prior to imaging.
BAF dimers (henceforth, "BAF") diffuse rapidly within living cell nuclei [25], [26].
We have therefore developed a labelling strategy for observation of single chromosomes in living interphase nuclei.
Diffusion of inert fluorescent proteins was studied in living cell nuclei using fluorescence correlation spectroscopy (FCS) with a two-color confocal scanning detection system.
In-vitro experiments provide evidence that this structure in turn condenses under certain salt conditions to an even more compact structure of 30 nm, but both its regularity and its existence in living cell nuclei are still under debate [2] [5].
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