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Data were quantified with the Living Imaging software by using absolute photon counts (photons/s) in an ROI, manually drawn to outline the BLI signal of the brain.
For bioluminescence quantification, regions of interest (ROI) were drawn by using the software Living Imaging 3.0 and average radiance (p/s/cm2/sr) was determined.
Interestingly, circulating cells of M12 variants displayed similar number patterns with the residual lung luminescence of living imaging.
Images were quantified using Living Imaging software (Xenogen) from a region of interest of the animal's ventral surface.
The growth and metastasis of tumor cells were monitored in real time with IVIS 200 imaging system, equipped with Living Imaging software (Xenogen, Alameda, CA).
Data quantification was analyzed using the IGOR-PRO Living Imaging Software (WaveMetrics, Inc., Lake Oswego, OR, USA).
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K.O., M.O., and T.S. contributed to live imaging.
All cells were maintained for 24 48 h post-transfection before fixation or live imaging.
Ratiometric live imaging of di-4-ANEPPDHQ detected membrane order levels in growing pollen tubes.
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