The liver was weighted and put into a manual glass homogenizer with lysis buffer (20 uL/mg), which was heated and then centrifuged to obtain supernatant for future enzymatic assays, according to the manufacturer-supplied instructions.
Briefly, liver samples were weighted, placed in iron-free Teflon vessels (ACV-Advanced Composite Vessel, CEM Corporation, Matthews NC, USA) and dried in a microwave oven (MDS 2000, CEM Corporation).
Specimens from the liver and kidney were weighted and homogenized separately with tissue homogenizer (MPW120, MPW Medical Instruments, China).
Three weeks following the final dose, the mice were euthanized and endometriotic lesions, whole body, liver, ovaries, uterine horn, and thymus were weighted, and lesion diameters were measured.
The brain, liver, spleen, heart and kidney harvested from the rats were weighted individually then examined macroscopically for any abnormality.
After 24 h of the last treatment, all the animals were weighted, sacrificed, collected the blood while liver was removed, weighted and perfuse in ice-cold saline solution.
+ Silymarin (100 mg/kg b.w. orally) Group VI: SME (200 mg/kg b.w. orally) alone After 24 h of the last treatment, all the animals were weighted, sacrificed, collected the blood while liver were removed, weighted and perfuse in ice-cold saline solution.
After 24 h of the last treatment, all the animals were weighted, sacrificed; collected their blood, weighted and perfuse a liver in ice-cold saline solution.
All categories were weighted equally.
