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Rat livers were weighed in a clean beaker.
Livers were weighed and homogenized in a buffer (pH 7.5) containing 250 mM sucrose, 1 mM EDTA and 10 mM Tris, with a final tissue concentration of 5%. 2 µl of plasma or liver homogenate was used to determine TG.
Livers were weighed and processed as described previously [ 12].
Livers were weighed, immediately frozen in liquid nitrogen, and stored at −80° for subsequent gene expression measurements.
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Testes and liver were weighed and quickly frozen on dry ice.
Following euthanasia whole spleen and liver were weighed; a section of each was weighed, homogenized and plated for bacterial CFU.
The uterus and liver were weighed and expressed on a per gram of body weight basis.
To determine the liver egg burden, each mouse liver were weighed, homogenized, and digested for approximately 1 h at 56°C with 10 mL 10% NaOH.
These fat depots, in addition to the liver, were weighed before being frozen in liquid nitrogen and stored at −80°C for later molecular analysis.
The hypothalamus was immediately frozen into liquid nitrogen and the liver was weighed.
The liver was weighed and the hepatosomatic index was calculated as the ratio of liver mass to body mass.
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