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Livers were snap frozen into liquid nitrogen for mRNA preparations.
Left lateral lobes of livers were snap frozen in liquid nitrogen and stored at -80°C.
Livers were snap frozen in liquid nitrogen and stored at –80°C.
Dissected livers were snap frozen in liquid nitrogen, sectioned into 10-μm sections, and processed for Oil Red O stain.
Male and female mice were killed at 3, 4, or 8 wk of age (n = 10-12 mice/sex/agroupoup) and livers were snap frozen in liquid nitrogen and stored at -80°C.
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In addition to the samples preserved in RNAlater, pieces of livers were snap-frozen in liquid nitrogen and stored at −80 °C for analysis of activities of 7-ethoxyresorufin-O-deethylase (EROD).
Livers were snap-frozen in liquid nitrogen immediately after harvest.
On days 24 and 25 (i.e. 2 3 days after the last injection), animals were sacrificed at the respective ZTs, and livers were snap-frozen in liquid nitrogen.
Livers were snap-frozen in liquid nitrogen and stored at −80°C or fixed in 4% formaldehyde.
Livers were snap-frozen in liquid nitrogen and stored at -80°C until use for transcriptomics and metabolomics analysis.
After 18 h, the animals were sacrificed; livers were snap-frozen in liquid nitrogen and stored at −80 °C until required.
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