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Their livers were separated about two weeks after they were born because their condition was deteriorating, but doctors held off doing more surgery to give them time to grow and become stronger.
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Nuclear matrices, prepared from human HepG2 cells and from rat liver, were separated by two-dimensional gel electrophoresis and were subsequently subjected to anti-TGF α immunoblotting; four spots around 17 kDa and an isoelectric point of 7.5 appeared.
Nuclei and cytoplasm (postnuclear supernatant) of human hepatoma cells and of rat liver were separated according to the method of Tata, applying 2.0 M sucrose for purification (Tata, 1974).
8 to 12 week old C57BL/6 J animals were euthanized using isoflurane, and the liver was collected in a Petri dish containing pre-warmed Williams E media The lobes of the liver were separated and were cut into small pieces of about 10 mm.
The herniated part of the liver was separated from the lung and returned to the abdominal cavity (Fig. 3c).
When the pressure-retrieved FFPE liver was separated by SDS-PAGE (Figure 3, lane 3), there were a number of well-resolved higher and lower molecular weight bands comparable to those seen in fresh liver extract (Figure 3, lane 1).
Data from patients who had surgical clips implanted (as a consequence of former partial liver resection) were separated from the patients without clips, and means were also compared separately for these two groups.
Liver leukocytes were separated by Ficoll density gradient centrifugation and splenocytes were prepared by mechanical disassociation.
Liver proteins were separated by large-gel 2-DE as described previously [44] [46].
Lipids from Percoll-purified liver mitochondria were separated into five phospholipid classes: phosphatidylcholine (PC), phosphatidylethanolamine (PE), cardiolipin (CL), phosphatidylserine (PS), and lysophosphatidylcholine (LYS).
Two enzymic activities in human liver homogenates were separated by ion exchange chromatography and the profile differed when activity was measured with p-AS and with sulphamethazine.
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