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Livers were sampled from carcasses intended for human consumption on nine hunting estates during two seasons (2012 13, 2013 14).
Livers were sampled on days 1, 4, 8 (sewage-exposed) and 16 (8 d of sewage exposure plus 8 d of recovery).
At the day 0 and eight additional time-points (day 1, day 3, week 1, 2, 4, 8, 12 and 16) mice were sacrificed, their livers were sampled and the RNA expression was analysed using NuGO Affymetrix mouse arrays [26].
TCDD was administered once orally at doses of 0, 1, 3, 10 and 30 μg/kg, and the livers were sampled 2, 4, 8 and 24 h after administration.
Livers were sampled from one male (104 cm; 18 kg) and one female (130 cm; 31 kg) adult broodstock maintained at St. Andrews Biological Station, St. Andrews, NB, Canada.
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Hypothalamus and liver were sampled on days 3 and 7 from the fasted fish, and from the fed fish at 3 h post-feeding.
The liver were sampled and stored at −80°C till use.
After the first week, five fish from each subgroup were weighed and muscle and liver were sampled.
For the present study brain, spleen, head kidney and liver were sampled.
For the congenic analysis, portions of liver were sampled at random for histology and for the recombinant analysis, liver tumors were randomly sampled.
At PND19 most pups were euthanized and the liver was sampled and frozen at -20°C until PCB analysis.
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