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Four to five livers were pooled for each RNA extraction.
We isolated HFLCs on three independent occasions; in each case four fetal livers were pooled.
In addition, for the purposes of considering gene expression polymorphism, the total RNAs from ten livers were pooled equally.
Since fetal livers were pooled, with male and female expected to be represented equally for each litter, a decision was made to also pool the postnatal livers with one male and one female per litter combined for RNA preparation.
Fetal livers were pooled by litter because these tissues were small and determination of sex of the fetuses was not feasible, as it would have required extensive additional work.
Similar(55)
The samples from incubation with rat liver microsomes were pooled into designed cassette groups and analyzed by HPLC/electrospray ITMS in full-scan mode.
The moose liver samples were pooled by the sampling area, sex and age.
In this estimation, reads from 11 liver samples were pooled together.
The light and heavy dimethyl-labeled peptides derived from equal amount of wild-type and HDAC6 knockout mouse liver protein extracts were pooled and desalted using C18 microcolumns.
Liver mononuclear cells were pooled prior to sorting.
The reads of brain and liver of each species were pooled and were assembled into unigenes.
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