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Livers were placed in Petri-dishes or on black tape to minimize light interference from plastic Petri-dishes.
Isolated perfused livers were placed in an NMR cell similar to that used for the NMR experiments.
To prepare precision-cut liver slices, the livers were placed into ice cold PBS for 10 min. Ten millimeter diameter tissue cores were prepared using a sharpened stainless steel tube.
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Following sacrifice, a portion of the epididymal adipose tissue and liver were placed in 10% formalin for 24 hours.
They were humanely killed and dissected; kidneys, lungs, heart, spleen, and liver were placed individually in 1.8-mL tubes and stored at −80°C.
One testis and a piece of liver were placed in separate cryovial tubes, snap-frozen in liquid nitrogen and then stored at −80°C.
After confirmation of euthanasia, a lateral incision exposed the peritoneal cavity and two lobes of the liver were placed onto sterile gauze over the animal's ribcage.
A mouse model of severe combined immunodeficiency disease (SCID) [ 27] was developed in which conjoint implants of human fetal thymus and liver were placed under the kidney capsule with subsequent infection of HCMV [ 28].
For Cd analysis, one kidney and a piece of liver were placed separately into sterile acid-washed microcentrifuge tubes (StarLab, Ahrensburg, Germany) and kept on dry ice before storage at −80°C.
Four cores of control tissue (2 from kidney, 2 from liver) were placed strategically in each tissue array to ensure unique orientation of every tissue array together with a maximum of 31 different patient tumor samples.
Three hours and 19 minutes after it had been taken out of Mr. Cawley, the liver was placed in Mrs. Hubbard.
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