All the meat offcuts (knuckle and face), and offal (tongue, heart, and liver) are mixed together with a load of fat.
All the meat offcuts (knuckle and face) and offal (tongue, heart, and liver) are mixed together with a load of fat.
For control reference, RNAs from normal liver tissues were mixed.
The livers (50 200 μg) were mixed in 1 mL of TRIzol® Reagent, using a Quiagen TissuLyser homogenizer; 200 μL of chloroform was added.
In brief, samples of plasma (200 μl) or liver (100 mg) were mixed with 2 ml of 1% pyrogallol (in ethanol) solution and saponified with 300 μl saturated sodium hydroxide solution.
Subsamples of blended tissues of pig liver and lungs were mixed with agar to a final concentration of 1% agar and the larvae allowed to migrate out of the agar-gel into 0.9% NaCl at 38°C.
Tissue extracts were prepared in a BeadBug microtube homogenizer D1030 (Benchmark Scientific, Edison, NJ, U.S.A .. Brain and liver (100 mg) were mixed with 400 μL of acetonitrile/water (3:1, v/v) containing 25 μg/mL BHT, a strong phenolic antioxidant, in a 2 mL homogenizer tube prefilled with zirconium beads (1.5 mm i.d).
For estimating the toxicity of Cd associated with the high molecular weight fraction, rat liver microsomes and CdCl2 were mixed with 1% of sodium deoxycholate and the protein-Cd complex produced was isolated by eluting with Sephadex G-75.
For PGE2 and soluble ICAM-1 (sICAM-1) analysis, liver slices (0.5 g) were mixed with 1.0 ml of homogenized buffer (0.25 mol/l sucrose, 50 mmol/l Tris HCl, and 5 mmol/l EDTA, pH 7.5).
Homogenates from either liver or brain (designated 14N liver and 14N brain) of rats were mixed at a 1∶1 wt/wt) ratio with a liver homogenate from a rat labeled with 15N enriched diet (designated 15N liver).
