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Livers were first perfused with perfusion medium (Gibco, Waltham, MA, USA, cat#17701-038) for 10 minutes at 5 ml/min.
Livers were first perfused in situ with 0.5 mM EGTA containing calcium-free salt solution, followed by perfusion with solution containing 0.02% collagenase D (Roche Diagnostics, Indianapolis, IN, USA).
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For detection of prothrombin, histologic sections of mouse liver were first blocked by using the avidin/biotin blocking kit (Invitrogen Corporation, Carlsbad, CA, USA).
For hepatic microsomes, liver was first perfused with 0.9% sodium chloride solution and excised out.
The perfused liver was first washed with solution I (8 g/l NaCl, 0.2 g/l KCl, 0.1 g/l Na2HPO4.12 H2.382.38 g/l HEPES, pH 7.6 and 0.5 mM EGTA) at a 10 mL/min flow rate for 8 10 min.
The abdomen was entered via an inverted T-shaped incision in the upper abdomen, and the RGEA graft on the left lobe of the liver was first identified.
CT volumetry of the liver was first performed on cadavers by Heymsfield et al. [4] in 1979 and was shown to be accurate within 5% of water displacement volumetry.
US of the liver was first performed with conventional B-mode.
In order to assess hepatic oxidative stress, the liver was first perfused with 10 mL of ice-cold 1.15% KCl solution through the portal vein.
Plasma was collected, and the pituitary gland, brain, and liver were dissected and frozen rapidly in liquid nitrogen, although the liver was first weighed to determine liver somatic index (LSI).
In Korea, cancer is the leading cause of mortality, and the liver is second most common primary tumor site that causes death.
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