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Lipids from livers were extracted using the Folch method [ 28].
Livers were extracted, and biochemical examination was performed.
Twenty four hours after slaughter, the fatty livers were extracted from the carcasses.
Fetal livers were extracted from embryos of the appropriate genotypes and single cell suspensions prepared in 600 μl of PBS.
Total lipid from the livers were extracted and dissolved in 200 μl of chloroform, 50 μl of which was loaded onto the TLC plates.
Bovine livers were extracted from animals and four cylindrical blocks of liver tissue with 90 mm in diameter and approximately 50 mm in thickness were dissected within 12 hour of sacrifice.
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Total RNA from murine livers was extracted using mirVana miRNA isolation kit (Ambion) without the miRNA enrichment step.
24 hours later, the animals were sacrificed and intestine and liver were extracted.
Total RNA from skeletal muscle (gastrocnemius), heart and liver was extracted by acid phenol extraction (Trizol, GIBCO/BRL) as recommended by the manufacturer.
The drug in blood and in liver was extracted and estimated by HPLC analysis at 4 hrs, 8 hrs and 16 hrs post administration.
Total RNA from mouse liver was extracted with TRIzol reagent, and purified and DNAse treated using the SV Total RNA Isolation System (Promega, Leiden, The Netherlands).
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