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Fish were carefully dissected, and whole livers were collected.
At the end of experiment, the livers were collected for histopathological analyses, protein extraction, and enzymatic activities.
To measure systemic levels of IFN activity for these mice, at the end of each experiment, livers were collected and gene expression of IFN-response genes was determined.
Study Design: Fifty fetal blood samples and 50 fetal livers were collected at 10 to 17 weeks' gestation and 8 to 17 weeks' gestation, respectively.
Caecum content and livers were collected and snap frozen in liquid nitrogen and stored at -80°C.
After 14 days, the body weight (grams) was measured, and then the mice were killed, the livers were collected and weighed (milligrams).
Blood and livers were collected.
Surviving mice (n = 3 5) were sacrificed after 44 hours and spleens and livers were collected.
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At 1 h p.i., the animal was sacrificed and blood, urine, kidneys, and liver were collected.
At 72 h after last administration, the kidney and liver were collected and fixed with 4% paraformaldehyde.
At 72 h after the last injection of vehicle or test article, the kidney and liver were collected.
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CEO of Professional Science Editing for Scientists @ prosciediting.com