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Dicer expression in primary MEFs and mouse livers was quantified via qRT-PCR using the iScript One-Step RT-PCR Kit with SYBR Green (BioRad) in accordance with the manufacturer's instructions.
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Histological sections of livers were quantified for liver metastasis (n = 34 53 fields).
The BLI signal of the liver was quantified from the fixed ROI on the dorsal image as demonstrated in Figures 1B and 1D.
The binding of NF-kB p50 subunit to DNA in mouse liver was quantified by ChIP-qPCR [ 10].
The total number of green fluorescent protein (GFP -positive nodules in the surface of all lobes of liver was quantified.
The image texture of the liver was quantified in regions-of-interest by calculating 165 texture features.
F-FDG uptake by the tumor xenografts, brain and liver was quantified by maximum standardized uptake values (SUVs) normalized to the weight of the animals.
Forty hours after sporozoite inoculation, the livers of all mice were harvested, total RNA was isolated using Tri-reagent (Molecular Research Center, Cincinnati, OH) and the parasite burden in the liver was quantified using reverse transcription (RT) followed by real-time PCR as outlined in Bruna-Romero et al. (2001).
MDA levels in the liver were quantified using a lipid peroxidation MDA assay kit (Beyotime Institute of Biotechnology, Jiangsu, China) according to the manufacturer's protocol.
After TAE treatment, CYPs activities in the rat liver were quantified using a cocktail of probe drugs and LC-MS/MS.
After 10 weeks, mice were euthanized, and metastatic nodules in lung and liver were quantified using dissecting microscopy after H&E staining.
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