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Hepatic hydroxyproline content was determined in analogue segments (50 mg) of snap-frozen livers using standard methods.
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Negative contrast TEM was performed on both SI contents and liver homogenate using standard protocols [ 19].
Genomic DNA was extracted from cell lines and liver metastases using standard genomic DNA isolation protocol.
Genomic DNA was prepared from 100-200 mg of spleen or liver tissue using standard phenol/chloroform extraction procedures.
This technique enables easy access to extracorporeal liver support by using standard dialysis devices [ 5].
The quantity of measured sterols (ng/mg liver) was calculated using standard curve, and these values were used for statistical analyses.
The blood sample is analysed for cholesterol (total, HDL & LDL), triglycerides, insulin, glucose and liver function indicators using standard automated techniques in that laboratory.
RNA was extracted, poly-A enriched, and cDNA libraries were prepared from A. contortrix and P. molurus liver tissue samples using standard techniques.
Using standard liver tests, a higher prevalence of "chronic liver disease" was observed in Italy, the USA and China, from 7.9%to17.5%5% of estimates [ 23- 26, 31].
Liver biochemistry was measured using standard laboratory methods (Roche modular system).
In the integrated database, liver biopsies were processed using standard techniques.
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