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Methionine was measured in extracts from Wt and Nox4-/ mouse livers using a Bridge-It® l-Methionine (l-Met) Fluorescence Assay (mediomics) as per the manufacturer's protocol.
We further demonstrated that this mechanism underlies hepatic fibrosis in animal models, as well as in patients, by detecting the N-terminal side LAP degradation products (…QILSKLR) ending at R residue (R LAP-DPs) in fibrotic livers using a specific antibody we generated [ 9].
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We confirmed the results of our FFPE HCC specimens and normal livers using an independent set of snap-frozen patient tissue.
Total RNA was extracted from all mouse livers using an RNeasy Mini kit and DNeasy Tissue kit (QIAGEN Inc., Valencia, CA, USA).
RNA was extracted from female livers using an RNeasy Mini extraction kit (Qiagen), incorporating on-column DNase treatment, according to the manufacturer's instructions.
a Image analyst roughly contours the liver using a cursor.
a, b Normal aspect of the liver using a transverse view at the anterior axillary line.
The present study investigated the effects of periodontitis on ethanol-induced oxidative damage of the liver using a rat model.
Geddes said the Norwegians' method involved a fiddly task of fishing out the liver using a net and poles.
The treatment procedure (TAOrMINA protocol) is characterised by the auto-transplantation and extracorporeal irradiation of the liver using a thermal neutron beam.
Aim of this study was to investigate if [11C]erlotinib PET is sensitive enough to measure a reduction of Abcg2 expression in the liver using a transgenic mouse model, in which the epidermal growth factor receptor (EGFR) was cell-type specifically deleted in hepatocytes of the liver leading to a down-regulation of Abcg2.
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