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For infection determination in vivo or ex vivo, total RNA was isolated from livers or primary hepatocytes using Qiagen's RNeasy Mini or Micro kits, respectively, following the manufacturer's instructions.
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Total RNA was isolated from epididymal adipose tissue, subcutaneous adipose tissue, liver, or primary macrophages using TRIzol reagent and protocol (Invitrogen).
We observe that CpG methylation of a canonical SP1, ETS, or NRF-1 site abolishes DNA binding of nuclear extracts isolated from either liver or primary keratinocytes.
Patients with underlying chronic liver disease or primary liver tumours were excluded from analysis.
Non-cirrhotic liver disease included all liver disease diagnoses except liver cirrhosis, eg, viral hepatitis, alcoholic hepatitis, non-alcoholic fatty liver disease, or primary biliary cirrhosis.
Nuclear and cytoplasmic fractions were obtained from fresh liver tissue or primary hepatocytes.
However, a major drawback of these studies is that they focused on selected etiologies, predominantly viral-related liver diseases or primary biliary cirrhosis [ 21- 24].
Sixteen out of 144 patients (11.1%) or 21 out of 151 transplantations (13.9%) (inclusive of four retransplantations) were high urgent listed and transplanted because of acute liver failure or primary graft nonfunction, respectively.
The S9 extracts are frequently sourced from rodents because of the limited availability of pathologically unaffected human liver tissue or primary human hepatocytes, which raises concerns about species specificity.
Age-matched mice were used to harvest liver or prepare primary hepatocytes.
The expression of miR-34a was markedly increased in alcohol-exposed liver or human primary hepatocytes.
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