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At first, by using a well-established intravenous infection model [35], we compared tissue burdens in kidneys and livers of groups of infected mice.
The present results were confirmed by absence of histopathological changes in livers of groups (2) and (4) in comparison with liver of control group.
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The %ID/g activity in the livers of group II animals is 42% of that in the group I animals at 3 hours post-injection, and 56% of that in the group I animals at 24 hours post-injection.
The main goal of this study was the identification of differentially-expressed genes in the liver of groups of Iberian x Landrace crossbred pigs showing extreme phenotypes for intramuscular fatty acid composition using RNA-Seq.
Histopathological analysis of liver cells using hematoxylin and eosin stains in Group III and Group IV rats treated with CCl4 revealed extensive liver damage, characterized by the disruption of the lattice nature of the hepatocytes, damaged cell membranes, degenerated nuclei, disintegrated central veins, and damaged hepatic sinusoids as compared to the liver of Groups I and II (control) rats.
The liver of group I showed normal hepatic parenchyma with no evidence of hepatocellular necrosis or inflammatory reaction (Fig. 4a).
The liver of group IV showed nearly similar picture to those demonstrated in the control one (Fig. 4h).
Meanwhile, the liver of group II revealed various histopathological alterations characterized by focal area of hepatocellular necrosis infiltrated by mononuclear cells (Fig. 4b) and polyploidy hepatocytes represented by hepatic cytokaryomegaly, binucleated hepatocytes associated with activation of Kupffer cells and sporadic cell necrosis (Fig. 4c) as well as apoptosis (Fig. 4d).
Sections from liver of group (4) which were sacrificed 15 dpc showed normal hepatic tissue.
The histologic injury scores in the liver of group 4 were significantly lower than those of group 3 (P < 0.05).
Gross examination of chicks liver of group B showed slight enlargement from the 1st week, whereas that of kidney from the 3rd week.
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