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In each experiment, n = 16 mice, and at each time point studied (2 hr and 12 hr postinfection), we isolated KCs from the livers of four mice treated with RPMI and four mice infected with L. donovani.
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We isolated total RNAs from frozen liver tissue using CsCl density-gradient centrifugation methods27.We pooled total RNA from the livers of ten wild-type mice and used them as reference in entire microarray experiments.
Analyses of the methylmercury levels according to Westöö6 in the livers of six seals shot in the German part of the Wadden Sea and two adults found dead showed that only 2 to 14% of the total mercury was methylmercury.
DNA microarray analysis was performed with total RNA samples from the livers of two toxic wild specimens in comparison with those from a nontoxic wild specimen and two nontoxic cultured specimens.
[Asish Chaudhuri, a biochemist at the VA Medical Center, in San Antonio] and colleagues made their discovery by extracting proteins from the livers of two long-lived bat species (Tadarida brasiliensis and Myotis velifer) and young adult mice and exposed them to chemicals known to cause protein misfolding.
A liver surgeon who branded his initials on the livers of two patients has been fined £10,000.
Two of the puppies were found to be bleeding internally and brodifacoum was detected in the livers of two of the dead puppies.
The museum's main attraction, however, is the infamous Si Quey, a Chinese immigrant who murdered and ate the hearts and livers of six Thai children in the late 50s.
The livers of five male pups from the transient hypothyroid model were analysed using Agilent gene expression microarrays alongside controls.
Total RNA was extracted from BAT or livers of three pairs of littermates (32 week-old) fed normal chow, using Qiagen RNeasy kit.
To test this hypothesis, we measured the expression levels of these eight genes in RNA samples from the livers of six human and six chimpanzee individuals, using quantitative RT-PCRs (see Materials and Methods).
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