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To identify biological pathways consistently modulated across multiple timepoints and various study designs, gene expression profiling was conducted on rat livers from three separate studies with triazole treatment groups ranging from 6 h after a single oral gavage exposure, to prenatal to adult exposures via feed.
The micrometastatic cells that were extracted to obtain RNA samples were captured by LCM from livers from three different areas (zones 1, 2 and 3).
We used livers from three individual fish from a monitoring station near OCSD outfall and four individuals from a monitoring station near the LACSD outfall for microarray analysis.
Adult mice (aged 20 to 36 weeks old) were anesthetized and livers from three mice finely minced in a sterile petri-dish.
To determine the amount of gene expression variability that exists between individuals of a single species, we compared the transcriptomes of livers from three fish with identical histories.
Gene expression changes were assessed in the livers from three mice in each of the 8 treatment groups in wild-type and PPARα-null mice using a total of 24 chips.
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a) W, Parp-+/+ livers from two animals (W1 & W2): H, Parp-1-/ livers from two animals (H1 & H2).
Normal human livers from ten patients were resected surgically because of hemangioma in liver in China.
Bisulphite sequencing was carried out on E14.5 livers from two wild-type (Rlf +/+ ) and two homozygous (Rlf MommeD28/MommeD28 ) littermates to an average depth of 30-fold per sample.
Livers from five KO and five WT mice were perfused in situ, via the hepatic vein, with cold phosphate-buffered saline to remove excess haemoglobin.
The technical replicate was obtained mincing fragments of livers from two hyperglycemic NOD mice and processing them as a completely independent sample.
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