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Total liver nucleated cell counts represented only 2% of wild-type livers at this stage (Fig. 2F).
Immunostaining with additional markers of cell cycle progression confirmed almost complete lack of mitosis in c-Met mutant livers at this time point (Figure 1C).
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GPX activity was similar in islets and liver at this age.
Heme biosynthesis was the second most significantly over-represented pathway, demonstrating the hematopoietic activity taking place in the embryonic liver at this stage.
This is in contrast to fetal SCN circadian rhythmicity which displays more distinct oscillations, suggesting a more immature nature of the molecular clock in the fetal liver at this developmental stage.
To determine the cellular content and structure of the human fetal liver at this early phase, we first conducted a histological analysis and investigated the expression pattern of known hepatocytes and cholangiocytes markers.
In our experiments 30%% was in the liver at this time (Table 3).
No mice showed symptoms of illness at 10 min post-injection (IP), and neither the WT D39 nor the IDM mutant strain was found in the heparinized blood or liver at this time point.
In this series of experiments we used BLI to measure reporter gene activity following biolistic transfer into either superficial (skin) or internal (liver) tissue in vivo, as shown in Figure 3. Peak BLI activity was observed at the 2d time-point following biolistic gene delivery into both tissues, with skin showing much greater BLI activity than liver at this point.
If you haven't already, it's usually common to remove the liver at this point to continue exploring the cavity inside.
Immunostaining for TBP or TAF4 in WT livers at the indicated stages (scale bar = 100 µm).
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