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These data provide a convenient method to isolate liver lymphocyte while preserving liver tissue for other analysis.
For example, the liver lymphocyte population includes both innate immune cells (such as the macrophages and natural killer cells) and adaptive immune cells (such as conventional T lymphocytes [ 14, 19] that recognize and respond to antigenic peptides presented by the major histocompatibility complex (MHC -I or -II [ 18, 20]).
All FPKM values for 5 human tissue-types (brain, liver, lymphocyte, lymphocyte XM, and lymphocyte XP) were filtered based on the GO-term of interest by mining the on-line AMIGO database for gene names associated with each Biological Process term of interest.
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We sought to determine whether these loci were associated with transcript levels in adipose, muscle, liver, lymphocytes, and pancreatic β-cells.
Our data demonstrated that liver lymphocytes can be efficiently isolated by simple perfusion.
Liver lymphocytes were prepared with the traditional method according to the methods of Matsuda with minor modified [4].
These cells comprise 20to35%5% of mouse liver lymphocytes and 10 to 10% of rat and human liver lymphocytes.
MTX has been shown to reduce intracellular folate levels in the liver, lymphocytes and RBCs [ 32, 33].
To assess tumor antigen specific CD8+ T cells, we stained isolated liver lymphocytes with an AH1 peptide loaded Ld tetramer.
Liver lymphocytes were co-cultured at a 1 1 ratio with YAC-1 target cells for 6 hr.
Flow-cytometric analysis also showed that Notch1 and FoxP3 dual expression was much higher in liver lymphocytes than peripheral lymphocytes of cirrhosis and HCC patients.
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