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Consistent with previous reports, pancreatic expression levels of the upstream transcript were higher than the downstream transcript [37], [38] and liver expression levels were higher for the downstream transcript than the upstream transcript [39].
It is likely that serological levels of IGF-1, IGFBP-3, and GHR are representative of the changes in liver expression levels, however further research is needed to confirm this assumption.
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Although three genes were not expressed in cultured liver cells, expression levels for the remaining 13 genes correlated with expression in the python liver, supporting the reproducibility of data across species despite the challenge of designing primers in an unannotated genome and measuring expression in the python.
OCT1 is mainly expressed in the liver, while expression levels in human cancers are variable (Hayer-Zillgen et al, 2002).
Analysis of liver MTP activity was consistent with the results of liver mRNA expression levels.
An analysis of the liver mRNA expression levels showed that no significant differences in the liver mRNA levels of SREBP-1c and PPAR-α1 were observed between the eNOS+/+ and HFD mice and the eNOS-/ and HFD mice.
In comparison to the esr1 mRNA in liver the expression levels were high in brain and testis (20 30% of the content in liver) whereas the expression levels of esr1 in eye, gill, intestine, swim bladder, kidney and ovary were lower (Figure 1A).
Although the initial (after a single dose) decrease in mRNA expression of the ET transporter OCTN1 in the liver, the expression levels returned to normal (same as controls) after 28 days.
In the liver Snca expression levels were similar in all three strains until day 9 when the expression was about two fold higher in A/J and BALB/c mice than in C57BL/6 mice (Fig 8a).
We next explored the effects of genetic variation on liver gene expression levels.
The liver mRNA expression levels of the genes involved in lipid metabolism are presented in Figure 2.
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