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In this study, two mutants of S. Choleraesuis were constructed and their attenuation and intracellular fate analysed with the purpose of engineering new attenuated live strains with improved properties as oral vaccine carriers.
Since the corresponding assay could be routinely and easily run by any microbiology laboratory, typing data from different groups could be merged and compared without the need to exchange live strains.
Some of these methods can be used in many laboratories to produce data which can be easily merged and compared without the need to exchange live strains, or other biological material.
Nonopsonic binding studies were performed with live strains of Mycobacterium bovis bacilli Calmette-Guérin (BCG) expressing either Green Fluorescent Protein (BCG-GFP) or bacterial luciferase (BCG-lux) as well as Mycobacterium tuberculosis H37Rv expressing bacterial luciferase [49] (Mtb-lux) and FITC-labeled zymosan (Molecular Probes).
These data indicate that a combination of three live strains of Lactobacillus (Lacto-mix), with evident inhibitory properties against EAE, is inducing a synergistic effect regarding the capacity to induce IL-10, suppression of the inflammatory cytokines and the therapeutic potential in diseased animals.
Live strains can colonize the gut, reducing the number of vaccine doses needed and significantly simplifying the immunization procedure.
Similar(51)
The Governor does not represent any live strain in conservatism; his gaze is backward.
The ability to feed live strain information from a structure back into the design cycle promotes refinement of the design itself, guidelines and methodology.
This is the first example of a P. mirabilis fimbrial antigen expressed in a food-grade live strain with promising applications in vaccine design.
Here a live strain of Pseudomonas aeruginosa was engineered to translocate a recombinant antigenic protein into mammalian cells via the type III secretion system, a bacterial device translocating effector proteins into host cells.
G3 was inoculated s.c. with 100 μL of the CP09 recombinant live strain (10 CFU).
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CEO of Professional Science Editing for Scientists @ prosciediting.com