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Visualization of mRNA dynamics in live neurons reveals its release from granules at synapses during neuronal plasticity.
STDP is a phenomenon discovered in live neurons by Bi and Poo [25] and adapted for learning event-based networks.
We measured the internal structure of the PSD in live neurons at approximately 25 nm resolution using photoactivated localization microscopy (PALM).
Valproate is known to injure neurons, so Cezar is converting embryonic stem cells into live neurons and adding valproate to the sample.
Here we report the development of a chamber that is able to be used for the continuous monitoring of live neurons under most commercially available upright epifluorescence and confocal microscopes and in which the temperature and composition of the medium surrounding the neurons can be changed rapidly and reversibly.
The two-photon fluorescent probe 2, which contains the di-2-picolylamine (DPA) as a Zn2+-binding site, the naphthalimide unit as the two-photon fluorophore, and the ifenprodil-like tail as the NMDA receptor binding moiety, can be employed for selective detection of Zn2+ located near the NMDA receptor and for monitoring concentration changes of Zn2+ in live neurons and hippocampal tissues.
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Circuit input includes state-variables of the live neuron.
Live neuron imaging requires the ability to work in significantly lower axial resolutions, with highly asymmetrical voxel dimensions.
In one of its implementations, a circuit injects currents to a live neuron through a microelectrode inserted into its soma, simulating a synaptic process [1].
The iPSC technology is an attractive approach to model a live neuron in neurological disorders.
The time- and labor-intensive nature of live neuron screening makes a higher sampling coverage impractical using current methods, and the limited lifetime of the dissociated OSNs precludes the testing of a larger group of compounds on a given OSN.
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