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The recovery was 89 97% and live lymphocytes were gated on forwarded versus sidescatter plot using flow cytometry.
CFSE dilution was analyzed in flow cytometry by gating on the live lymphocytes and proliferation was quantified the ModFit software (Verity Software House, Topsham, ME).
Live lymphocytes were gated by Forward Scatter (FSC) / Side Scatter (SSC) profiles.
Live lymphocytes were gated on FSC/SSC plots, and the percentage of FOXP3+ CD4+ T cells was assessed.
Cells were cultured for 48 h, then BAFF-R expression on B cells was analyzed by FACS gating on live lymphocytes.
Second, spleen cells from acutely arthritic (AA) donor mice were stimulated in vitro with cartilage PG, and live lymphocytes were isolated on a Lympholyte-M density gradient.
Similar(48)
Events in a live lymphocyte gate were analyzed with CellQuest (BD Biosciences) software.
Fifty thousand events (proliferation evaluation) and 100,000 events (memory phenotype) were acquired in a live lymphocyte gate.
Forward/side scatter dot plot was used to gate the live lymphocyte population.
Samples were run for a minimum of 300,000 events in a live lymphocyte gate.
The live lymphocyte gate (at least 1,000,000 live events) was set based on forward and side scatter for further analysis.
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