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These methods can be readily extended to identifying and sorting live embryonic stem cells, which could automate and improve the procedure for separating out stem cells that remain pluripotent from those that have begun to differentiate.
The dynamics of the motion of each cardiac cell was imaged by a line-scanning high-speed confocal microscopy system, which was previously used for imaging the live embryonic heart of zebrafish [6].
In our recent work (Chen et al., 2014b), we described a single-cell, single-molecule imaging strategy to study the in vivo Sox2 and Oct4 target search process and dissect the kinetics of enhanceosome formation at endogenous single-copy gene loci in live embryonic stem (ES) cells.
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The abortion foes accused Mr. Bush of breaking a campaign promise to outlaw research on living embryonic cells, a pledge he reiterated to an anti-abortion group in May.
To meet these demands, cell-derived nanovesicles that mimic exosomes were generated by extruding living embryonic stem cells through micro-filters.
Now Liu et al. have taken advantage of advanced microscopes to observe the interaction between Sox2 and its binding site in the nucleus of living embryonic cells.
Here, we combine lattice light-sheet imaging, single-molecule tracking, numerical simulations, and ChIP-exo mapping to localize and functionally probe Sox2 enhancer-organization in living embryonic stem cells.
The results demonstrated that ZnS and Zn0.5Cd0.5S conjugates were non-toxic and behaved as effective fluorophores for in vitro imaging live human embryonic kidney cells (HEK293T) with confocal fluorescence microscopy.
The multimodal measurements are here illustrated by measuring live mouse embryonic fibroblasts cells (MEF) [18], cultured in Dulbecco's modified Eagle medium, and plated on quartz-bottom dishes one day prior experiment.
Such markers are still scarce in Drosophila but are crucial to conducting cell biology studies in live tissues, embryonic or other.
Hence, it may be surmised that tumor cells –alike embryonic tissues– live at a very low [H2O2]ss [10] [13], [13].
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