Approximately 23 different species in North America emerged in as little as 1-3 million years [ 18] resulting in little sequence variation in DNA regions such as chloroplast and rDNA [ 15].
In Saccharomyces cerevisiae, ORC binds to specific DNA elements; however, in higher eukaryotes, ORC exhibits little sequence specificity in vitro or in vivo.
The alignments between L. luteus and Medicago showed high levels of conservation in the coding regions, but little sequence similarity in the intergenic regions.
In both mouse and human, β-defensin paralogues show little sequence similarity in the mature peptide region and this divergence appears to have been driven by positive selection following duplication [ 9, 10].
Both IL-3 and GM-CSF have an extended loop between helix A and B but share little sequence conservation in this region (Figure 1C), suggesting that interface V can form in the two family members but not between them.
This region is unique since there is little sequence homology in this region.
Consistent with the evidence that there was little sequence variability in the chCaRE sequence at positions 3, 6, 7, and 8 (Figure 5a) switching the base at any of these positions essentially eliminated the ability of the altered oligo to compete the binding of CaRF to the camCaRE (Figure 5c).
All three proteins have single predicted amino-terminus transmembrane domains despite having very little sequence identity in this region.
Although there was relatively little sequence diversity in the housekeeping genes, the number of STs (0.51 ST/isolate) was similar to that of other bacterial pathogens.
