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"It's hard to estimate the volume of litter generated, but it's significant".
After the show the clear-up begins, but what happens to the tonnes of litter generated by Glastonbury's huge crowds?
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Polymerase chain reaction (PCR) genotyping of litters generated from crosses of heterozygotes resulted in the expected amplification products (Fig. 1D).
For each array hybridization, the paired DNA samples used were matched between pups of litters generated by pair-fed saline-control and cocaine-treatment dams.
As we have described previously [ 12], we established eight selection lines by recruiting animals from 60 litters generated by a colony of wild-type mice maintained at the Animal Resources Centre (Murdoch, Western Australia).
Wheareas overt forms were sporadic, the dissection of several litters generated from NHERF1 heterozygous parents showed a variable penetrance of the mild phenotype with rates up to 100% (Additional file 1: Figure S1B).
The litter cover generated the highest subsurface flow rate, followed by the grass cover; the lowest was on bare land.
A total of 248 ORF5 sequences from 235 litters and 288 ORF7 sequences from 266 litters were generated.
In addition, eight intercross litters were generated (B6xD2J F1s x B6xD2 F1s) with parents heterozygous at the mutant locus.
Thirteen contemporary litters were generated (ten of purebred and three of crossbred animals).
FVBN (Taconic) wild-type mouse litters were generated from timed matings, where presence of the copulation plug was considered E0.5.
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