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In the second stage a household listing was performed within the cluster and a systematic sample of 30 households was drawn to obtain 1,200 households per district.
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An analysis of the costs of the simulated extra lists was performed by the finance department (Table 3).
No prioritization within the gene list was performed.
Smoothing of the peak list was performed using the Savitzky Golay method to 2 smooths.
The data normalisation and statistical analysis to generate E-dependent DEG list was performed by the AGRF Melbourne, Australia.
Functional analysis of gene lists was performed in DAVID [21], [88], using the PANTHER functional annotation classes.
The integration of significantly hypo- and hyper-methylated, over- and under-expressed, and gained and lost gene lists was performed using the VENN tool in PGS, and visualization to the resulting tracks was performed as previously described [39].
Global literature analysis of various gene lists was performed using BiblioSphere PathwayEdition (Genomatix Software GmbH, Munchen, Federal Republic of Germany) software which performs pathway and interaction analysis and labels genes which belong to certain known metabolic and signal transduction pathways.
Annotation of monoisotopic peptide signals in the m/z range 800 4000 by the SNAP algorithm (S/N threshold = 4, quality factor threshold = 50, number of peaks limited to 100) and generation of the corresponding peak lists was performed with the FlexAnalysis 3.0 post-processing software (Bruker Daltonics).
Functional analysis of gene lists was performed by Ontologizer 2.0 (Bauer et al., 2008).
Functional enrichment analysis of gene lists was performed using the DAVID webtool [ 53, 54].
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