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Table 2 List of constructed yeast strains Strain name Feature Hyn B Displaying Hyn B on the cell membrane Ni Hyd Displaying Ni Hyd on the cell membrane control Displaying only FLAG tag as a negative control for platinum reduction.
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Proteins were expressed in Escherichia coli strain BL21 STAR (Invitrogen) (see Figure 1A for complete list of constructs).
List of constructs.
Supplementary file 1 shows a full list of constructs used.
A full list of constructs used in this study is given in Table S1.
A list of constructs is provided in supplementary material Table S2.
Therefore, we developed a coding scheme comprising an exhaustive list of constructs in an iterative manner, adding new constructs as we encountered them.
Then the list of constructs became theoretically saturated [ 32].> The researchers stayed in the setting over time thus enabling interpretation of the meaning in individuals' lives [ 34].
A list of constructs and in situ probes of the Zebrafish genes generated in this work, containing primers and amounts injected, is provided in Supplementary file 1B.
The LLG1 promoter::GUS (pLLG1 GUS) expression pattern overlapped considerably with that of pFER::GUS (Duan et al., 2010) in Arabidopsis seedlings (see Supplementary file 1 for a list of constructs used).
A list of constructs is shown in Supplementary file 1; domain maps for some are shown in Figure 8. Plant genomic DNA was used for PCR analysis of T-DNA inserts in transformed plants.
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