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The experiment was conducted over 80 days in four 20 day periods that consisted of 14 days of acclimatization, 5 days of measurement and 1 more day for rumen liquor sample collection.
At the conclusion of each experiment, a liquor sample was removed, filtered, and analyzed for monomeric sugars.
The calculated results of the enzymatic hydrolysis experiments were determined from knowledge of the initial dry weight of biomass and the sugar content of the liquor sample.
On the one hand, a liquor sample of 3 mL was post-hydrolyzed with 4%H2SO44 at 121°C for 1 h to determine the total concentration of XOS and their degree of substitution by acetyl group.
For LC MS analysis, 10 µL of undiluted pretreatment liquor sample was adjusted to pH 2.0 with formic acid and was injected into a XEVO G2SQTOF mass spectrometer in combination with a Waters Acquity UPLC system, and equipped with an ESI interface capable of operating in both positive- and negative-ion modes.
In the third liquor sample, after the patient received appropriate antbiotic therapy, CXCL13 levels have already been decreased to 450 pg/ml [see Table 1 and CSF cell count dropped to 190 cells. However, cranial nerve palsy was persisting, without signs of clinical improvement.
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The optimized method was then applied to cocoa liquor samples of Ghana and Ecuador, produced with different roasting temperatures in an Italian industrial plant.
Each experimental period lasted 16 days: 8 days for adaptation to the diet and 8 days for total feces collection and rumen liquor sampling; urine was collected the last two days.
Historically investigation into activated sludge foaming has involved either measuring, under aeration conditions, the propensity of mixed liquor samples to foam, or evaluating different physico-chemical properties of the sludge which have previously been linked to activated sludge foaming.
Figure 2 A schematic of the industrial washing machine used and place of liquor sampling.
Then, mixed liquor samples from the bioreactor were monitored for pH and COD analysis after the experiment (APHA 2012).
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